鸡Heme oxygenase 1基因克隆、结构功能预测及组织表达特性分析

doi:10.11843/j.issn.0366-6964.2014.01.005

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (1): 31-37.doi: 10.11843/j.issn.0366-6964.2014.01.005

鸡Heme oxygenase 1基因克隆、结构功能预测及组织表达特性分析

江秀清¹，王丽丽¹，曹嫦妤1，李楠¹，葛延松¹，张颖^2*，李金龙^1*

（1.东北农业大学动物医学学院，哈尔滨 150030； 2.东北农业大学资源与环境学院，哈尔滨 150030）

收稿日期:2013-06-26 出版日期:2014-01-23 发布日期:2014-01-23
通讯作者: 张颖，教授，E-mail：zhangyingghr@hotmail.com；李金龙，副教授，E-mail：jinlongli@neau.edu.cn
作者简介:江秀清(1990-)，女，河南杞县人，硕士生，主要从事动物营养代谢病与中毒病，环境毒理学研究，E-mail：ja9075@163.com；王丽丽(1986-)，女，河北沧县人，硕士生，主要从事动物营养代谢病与中毒病，环境毒理学研究，E-mail：iamwanglili1003@163.com
基金资助:
国家自然科学基金青年基金项目(30901088)；教育部新世纪优秀人才培养计划(NECT-1207-02)；黑龙江省普通高等学校新世纪优秀人才培养计划(1252-NCET-009)；中国博士后科学基金项目(2012T50301和20110491021)；黑龙江省教育厅科学技术研究项目(12511031)；国家科技重大专项项目（2012ZX07201003）

Gene Cloning，Analysis of Bioinformatics and Tissue Specific Expression Profile of Heme Oxygenase 1 Gene in Chicken

JIANG Xiu-qing¹，WANG Li-li¹，CAO Chang-yu¹，LI Nan¹，GE Yan-song¹，ZHANG Ying^2*，LI Jin-long^1*

(1.College of Veterinary Medicine，Northeast Agricultural University，Harbin 150030，China；2.College of Resources and Environment, Northeast Agricultural University,Harbin 150030，China)

Received:2013-06-26 Online:2014-01-23 Published:2014-01-23

摘要/Abstract

摘要：

旨在克隆鸡Heme oxygenase 1（HO-1）基因，探明其蛋白结构、功能及组织表达特性。以海兰白鸡（W-36）为材料，利用RT-PCR技术克隆鸡HO-1基因CDS区，利用生物信息学方法预测蛋白结构与功能，应用qRT-PCR检测组织表达特性。结果表明，鸡HO-1基因的CDS为891 bp，编码296个氨基酸，其核苷酸和氨基酸序列与其他种属间的差异较大。鸡HO-1为跨膜非分泌蛋白，具有HO家族的结构域，酶分类为EC1.14.99.3，能够结合HEM、BLA、OXN、Q80、SUC、TRE和Na⁺等分子。鸡HO-1基因在组织内广泛表达，在动脉、脑干、大脑、坐骨神经、十二指肠及小肠等组织表达量较高。预测HO-1蛋白具有调控糖类和盐类等相关分子功能作用，其在组织中广泛表达，在循环系统、神经系统和消化系统中具有重要的生物学功能。

Abstract:

The aim of this study was to clone the chicken Heme oxygenase 1 (HO-1) gene，predict its protein structure and function，and reveal its expression characteristics in tissues.In this study，Hailan White fowl (W-36) was used as experimental animal，the CDS region of chicken HO-1 gene was cloned by qRT-PCR，the protein structure and function were predicted by using bioinformatics，RT-PCR was used to analyze the expression characteristics in tissues.These results showed that the CDS of chicken HO-1 gene was 891 bp，encoding 296 amino acids.The conservation of its nucleotide and amino acid sequences were not high compared with other species.The protein of chicken HO-1 was transmembrane and non-secretory，it had a domain of the HO family，it is classified in EC1.14.99.3.It could bind to HEM，BLA，OXN，Q80，SUC，TRE and Na⁺ molecules，the HO-1 was expressed widely in tissues，and the expression level was higher in the brain，brain stem，artery，sciatic nerve，duodenum and small intestine.HO-1 protein is predicted that it can regulate the sugar and salt function，and is expressed widely in tissues，with important biological function in the circulatory system，nervous system and digestive system.

中图分类号:

江秀清，王丽丽，曹嫦妤，李楠，葛延松，张颖，李金龙. 鸡Heme oxygenase 1基因克隆、结构功能预测及组织表达特性分析[J]. 畜牧兽医学报, 2014, 45(1): 31-37.

JIANG Xiu-qing，WANG Li-li，CAO Chang-yu，LI Nan，GE Yan-song，ZHANG Ying，LI Jin-long. Gene Cloning，Analysis of Bioinformatics and Tissue Specific Expression Profile of Heme Oxygenase 1 Gene in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 31-37.

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鸡Heme oxygenase 1基因克隆、结构功能预测及组织表达特性分析

Gene Cloning，Analysis of Bioinformatics and Tissue Specific Expression Profile of Heme Oxygenase 1 Gene in Chicken

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